Gabapentin Forensic ELISA Kit

SKU 编号  182419  |  目录编号 

  • Highly sensitive
  • Multiple matrices
  • Large menu of tests available
数量:
最低数量要求:1
This item must be ordered in multiples of one case. Quantity per case: 1
Neogen’s Gabapentin ELISA (Enzyme-Linked ImmunoSorbent Assay) test kit is a qualitative kit designed for use as a screening device for the detection of drugs and/or their metabolites. The kit was designed for screening purposes and is intended for forensic use only.
规格
品牌Neogen®
分析物Gabapentin
应用尿, 血, 口腔液, 取证矩阵
预期用途For the determination of trace quantities of Gabapentin and/or other metabolites in urine, blood, serum and oral fluid. Contact a Neogen representative for information on other matrices.
物种人, 研究动物
Assay Sample SizeOral Fluid: 50 µL
Blood, Serum, Urine: 10 µL
平台酶联免疫吸附
结果类型定性
微孔板尺寸96 well microplate
敏感性
CompoundI-50 in EIA BufferI-50 in Whole Blood
(1:20 Dilution: 1 part sample to 19 parts EIA buffer)
I-50 in Serum
(1:20 Dilution: 1 part sample to 19 parts EIA buffer)
I-50 in Urine
(1:20 Dilution: 1 part sample to 19 parts EIA buffer)
I-50 in Oral Fluid
(1:4 Dilution: 1 part sample to 3 parts Oral Fluid Buffer*)
Gabapentin11 ng/mL676 ng/mL735 ng/mL755 ng/mL100 ng/mL

*Oral Fluid Buffer is sold separately.

The term I-50 is used to define the sensitivity of the test. This number is derived from a standard curve generated with the drug in EIA Buffer. The drug concentration that shows 50% less color activity than the zero standard is considered to be the I-50.

总检测孵育时间75 minutes
药物面板处方药, 工作场所测试
储存条件Test kit 2-8°C; store controls -20°C
波长450 nm with acid stop
每个包裹的数量96 wells
Compound% Cross Reactivity
Gabapentin100
Pregabalin methyl ester9.4
(-)-Levamisole4.5
Gabapentin Enacarbil4.3
Pyrantel1.9
Pregabalin1.1
R(-)-Methamphetamine0.1
  1. EIA Buffer
  2. Wash Buffer Concentrate
  3. K-Blue Substrate
  4. Drug-Enzyme Conjugate
  5. Drug-Enzyme Diluent
  6. Antibody Coated Plate
  7. Acid Stop Solution
  8. Qualitative QC Positive Control
  9. Qualitative QC Negative Control
  1. Deionized water.
  2. Precision pipettes that range from 10 μL - 1000 μL and disposable tips.
  3. Graduated cylinder to dilute and mix wash buffer.
  4. Plate cover or plastic film to cover plate during incubation.
  5. Clean glassware (i.e. test tubes) to dilute samples.
  6. Microplate reader with 450 nm filter.

科技

ELISA Assay Theory

Neogen’s direct competitive ELISAs operate on the basis of competition between the horseradish peroxidase (HRP) enzyme conjugate and the analyte in the sample for a limited number of specific binding sites on the precoated microplate.

ELISA Assay Procedure

Samples, standards or calibrators are first added to the precoated antibody microplate. Next, the enzyme conjugate is added and the mixture is incubated at room temperature. During incubation, competition for binding sites on the microplate is taking place. The plate is then washed, removing all unbound material. The bound enzyme conjugate is detected by the addition of a TMB-based substrate.

ELISA test results may be obtained by measuring and comparing the absorbance reading of the wells of the samples against the standards with a microplate reader at 650 nm or 450 nm if acid stop is used. The extent of color development is inversely proportional to the amount of analyte in the sample or standard.

ELISA Assay Test Principle Procedure

  1. Plates are precoated with the antibody. The plate is ready for use. DO NOT WASH.
  2. A sample or control is added to each well. Next the drug-enzyme conjugate is added and the mixture is incubated at room temperature.
  3. Wash the plate to remove all unbound materials.
  4. The bound materials now remain in the microplate.
  5. Add TMB substrate to each well and allow the color to develop.
  6. Qualitative results are obtained by measuring the absorbance reading at 650 nm or 450 nm if acid stop is used.
AssayPrincipleIllustration_Forensic.png

培训

Our customers’ success is our shared success. Our experts are ready to train you and your team on our solutions, so you can rest easy knowing procedures are performed properly and yield accurate results. In addition, we provide certificates upon completion of training.