Neogen® Molecular Detection System™ Technology

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Make the Move to LAMP

A Modern Molecular Method, Built on LAMP Technology

Loop-mediated isothermal DNA amplification (LAMP) technology uses strand-displacing Bst DNA polymerase and four to six primers to produce continuous DNA amplification at a single temperature.

Traditional polymerase chain reaction (PCR) technology uses a non-strand displacing Taq DNA polymerase, thermocycling, and two primers. Bst polymerase used in LAMP is more robust and less prone to inhibition than Taq polymerase.

PCR technology uses an internal amplification control (IAC) while LAMP technology does not utilize an IAC since it is not subject to the same limitations as PCR.

Benefits of LAMP technology used in the Neogen Molecular Detection System for pathogen testing.

Make the move to LAMP

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Amplification Technology
PCR
PCR
(Polymerase Chain Reaction)
Traditional | Complex
LAMP
MDS LAMP
(Loop-Mediated Isothermal DNA Amplification)
Progressive | Simplified
Reaction Robustness
Thermal Cycling
Provides opportunities for inhibitors to interfere.
Continuous Amplification
Excellent tolerance to common PCR inhibitors.
Preparation
Requires Preparation
Lysis buffers are not pre-dispensed, increasing complexity, labor, and cross-contamination risk.
Ready to Use
No buffers or reagent mixes to prepare with no risk of false negative due to incorrect preparation.
Workflow
Multiple Protocols
Different test protocols for each pathogen increase complexity and risk of error.
Single Protocol
One process for all pathogens streamlines workflow and reduces risk.
Controls
IAC Needed
Internal amplification control (IAC) is necessary to overcome inherent limitations/challenges.
IAC Not Needed
Matrix Control and Reagent Controls provide additional assurance.

MDS100: A Compact Instrument

  • Space-saving design fits in any lab
  • Flexibility to test up to 96 samples in each run
  • Quick, compliant, cost-saving calibration—just send your unit to our facility for expert service
  • Global service with local support teams

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Lab technician runs pathogen tests on a compact Neogen Molecular Detection Instrument using LAMP.
Lab technician runs pathogen tests on a compact Neogen Molecular Detection Instrument using LAMP.

User-Friendly Software

  • User-friendly software with intuitive user interface for quick and easy setup
  • Real-time results help make critical decisions faster
  • Color-coded assays by organism and matching software screens allow for better tracking and easy identification
  • Ability to run simultaneously with other qualitative assays
  • Flexibility to test up to 96 samples in each run

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Lab technician checks real-time LAMP pathogen test results on Neogen Molecular Detection System software.
Lab technician checks real-time LAMP pathogen test results on Neogen Molecular Detection System software.
Lab with the Neogen Molecular Detection System running LAMP tests, as shown in QuanTEM case study.
Lab with the Neogen Molecular Detection System running LAMP tests, as shown in QuanTEM case study.

Why QuanTEM Food Safety Laboratories Switched to LAMP

Check out our blog, "Using Neogen® Molecular Detection System: Q&A with QuanTEM Food Safety Laboratories," to learn more about the technology.

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